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admscs  (ATCC)


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    ATCC admscs
    Admscs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mesenchymal+stem+cells+admsc/ASC52telo%2C+hTERT+immortalized+adipose+derived+Mesenchymal+stem+cells/pmc13088179-38-1-4
    Average 96 stars, based on 250 article reviews
    admscs - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Bioinspired short peptide hydrogel for versatile encapsulation and controlled release of growth factor therapeutics.
    Article Snippet: A short bioinspired octapeptide, GV8, can self-assemble under mild conditions into biodegradable supramolecular physical hydrogels with high storage modulus and good biocompatibility.. GV8 hydrogels can encapsulate both single or multiple macromolecular protein-based therapeutics in a simple one-pot formulation manner, making it a promising candidate to address challenges faced by existing synthetic polymer or peptide hydrogels with complex gelation and drug-encapsulation processes.. Alongside its versatility, the hydrogel exhibits concentration-dependent storage modulus and controlled drug-release action.

    Article Title: Highly variable biological effects of statins on cancer, non-cancer, and stem cells in vitro.
    Article Snippet: The human pancreatic cancer cell line MiaPaCa-2 (ATCC, Manassas, VA, CRL-1420, LOT 70014313, RRID: CVCL_0428) and human embryonic kidney cell line HEK 293 (ATCC, Manassas, VA, CRL-1573, LOT 70039815, RRID:CVCL_0045) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Sigma Aldrich, Germany) supplemented with 10% fetal bovine serum. .. Human adipose-derived mesenchymal stem cells ADMSC (ATCC, Manassas, VA, PSC-500-011, LOT 70017032, positive specific staining for CD29, CD44, CD73, CD90, CD105, and CD166 and negative for CD14, CD31, CD34, and CD45) were cultured in mesenchymal stem cell basal medium (ATCC, Manassas, VA) supplemented with low serum mesenchymal stem cell growth kit for 9 Vol. .. :(0123456789) Scientific Reports | (2024) 14:11830 | https://doi.org/10.1038/s41598-024-62615-w adipose- and umbilical-derived MSCs (ATCC, Manassas, VA).

    Article Title: Highly variable biological effects of statins on cancer, non-cancer, and stem cells in vitro
    Article Snippet: The human pancreatic cancer cell line MiaPaCa-2 (ATCC, Manassas, VA, CRL-1420, LOT 70014313, RRID: CVCL_0428) and human embryonic kidney cell line HEK 293 (ATCC, Manassas, VA, CRL-1573, LOT 70039815, RRID:CVCL_0045) were cultured in Dulbecco's Modified Eagle Medium (DMEM; Sigma Aldrich, Germany) supplemented with 10% fetal bovine serum. .. Human adipose-derived mesenchymal stem cells ADMSC (ATCC, Manassas, VA, PSC-500-011, LOT 70017032, positive specific staining for CD29, CD44, CD73, CD90, CD105, and CD166 and negative for CD14, CD31, CD34, and CD45) were cultured in mesenchymal stem cell basal medium (ATCC, Manassas, VA) supplemented with low serum mesenchymal stem cell growth kit for adipose- and umbilical-derived MSCs (ATCC, Manassas, VA). ..

    Staining:

    Article Title: Highly variable biological effects of statins on cancer, non-cancer, and stem cells in vitro.
    Article Snippet: The human pancreatic cancer cell line MiaPaCa-2 (ATCC, Manassas, VA, CRL-1420, LOT 70014313, RRID: CVCL_0428) and human embryonic kidney cell line HEK 293 (ATCC, Manassas, VA, CRL-1573, LOT 70039815, RRID:CVCL_0045) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Sigma Aldrich, Germany) supplemented with 10% fetal bovine serum. .. Human adipose-derived mesenchymal stem cells ADMSC (ATCC, Manassas, VA, PSC-500-011, LOT 70017032, positive specific staining for CD29, CD44, CD73, CD90, CD105, and CD166 and negative for CD14, CD31, CD34, and CD45) were cultured in mesenchymal stem cell basal medium (ATCC, Manassas, VA) supplemented with low serum mesenchymal stem cell growth kit for 9 Vol. .. :(0123456789) Scientific Reports | (2024) 14:11830 | https://doi.org/10.1038/s41598-024-62615-w adipose- and umbilical-derived MSCs (ATCC, Manassas, VA).

    Article Title: Highly variable biological effects of statins on cancer, non-cancer, and stem cells in vitro
    Article Snippet: The human pancreatic cancer cell line MiaPaCa-2 (ATCC, Manassas, VA, CRL-1420, LOT 70014313, RRID: CVCL_0428) and human embryonic kidney cell line HEK 293 (ATCC, Manassas, VA, CRL-1573, LOT 70039815, RRID:CVCL_0045) were cultured in Dulbecco's Modified Eagle Medium (DMEM; Sigma Aldrich, Germany) supplemented with 10% fetal bovine serum. .. Human adipose-derived mesenchymal stem cells ADMSC (ATCC, Manassas, VA, PSC-500-011, LOT 70017032, positive specific staining for CD29, CD44, CD73, CD90, CD105, and CD166 and negative for CD14, CD31, CD34, and CD45) were cultured in mesenchymal stem cell basal medium (ATCC, Manassas, VA) supplemented with low serum mesenchymal stem cell growth kit for adipose- and umbilical-derived MSCs (ATCC, Manassas, VA). ..



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    Functional screening of drugs promoting chondrogenic differentiation ( A ) First Screening of Cartilage Differentiation-Promoting Drugs: Adipose-derived <t>mesenchymal</t> stem cells <t>(ADMSCs)</t> were treated with 10 µM of each drug for 72 h. The expression levels of COL2A1 (top) and SOX9 (bottom) mRNA were assessed using qRT-PCR. Dotted lines indicate drugs that significantly increased SOX9 and COL2A1 expression compared to DMSO control. ( B ) Second Screening: The Venn diagram shows the overlap of drugs that increased COL2A1 and SOX9 expression. Bar graphs present relative mRNA expression levels with statistical significance indicated. The cartilage-differentiation effect of seven drugs selected from the first screening was re-evaluated by measuring changes in mRNA levels of COL2A1, SOX9, and aggrecan (ACAN).: * P < 0.05, ** P < 0.01, *** P < 0.001 ( n = 3). ( C ) ADMSCs were cultured in pellet form and treated with DMSO, aripiprazole, or irinotecan for three weeks. Aripiprazole treatment showed increased cartilage differentiation compared to DMSO and irinotecan. ( D ) Hematoxylin and Eosin (H&E) staining and Alcian blue staining of pellet cultures. Aripiprazole treatment resulted in larger pellet size and more intense staining, indicating enhanced cartilage matrix production. Scale bars represent 100 μm.
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    Functional screening of drugs promoting chondrogenic differentiation ( A ) First Screening of Cartilage Differentiation-Promoting Drugs: Adipose-derived <t>mesenchymal</t> stem cells <t>(ADMSCs)</t> were treated with 10 µM of each drug for 72 h. The expression levels of COL2A1 (top) and SOX9 (bottom) mRNA were assessed using qRT-PCR. Dotted lines indicate drugs that significantly increased SOX9 and COL2A1 expression compared to DMSO control. ( B ) Second Screening: The Venn diagram shows the overlap of drugs that increased COL2A1 and SOX9 expression. Bar graphs present relative mRNA expression levels with statistical significance indicated. The cartilage-differentiation effect of seven drugs selected from the first screening was re-evaluated by measuring changes in mRNA levels of COL2A1, SOX9, and aggrecan (ACAN).: * P < 0.05, ** P < 0.01, *** P < 0.001 ( n = 3). ( C ) ADMSCs were cultured in pellet form and treated with DMSO, aripiprazole, or irinotecan for three weeks. Aripiprazole treatment showed increased cartilage differentiation compared to DMSO and irinotecan. ( D ) Hematoxylin and Eosin (H&E) staining and Alcian blue staining of pellet cultures. Aripiprazole treatment resulted in larger pellet size and more intense staining, indicating enhanced cartilage matrix production. Scale bars represent 100 μm.
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    Functional screening of drugs promoting chondrogenic differentiation ( A ) First Screening of Cartilage Differentiation-Promoting Drugs: Adipose-derived mesenchymal stem cells (ADMSCs) were treated with 10 µM of each drug for 72 h. The expression levels of COL2A1 (top) and SOX9 (bottom) mRNA were assessed using qRT-PCR. Dotted lines indicate drugs that significantly increased SOX9 and COL2A1 expression compared to DMSO control. ( B ) Second Screening: The Venn diagram shows the overlap of drugs that increased COL2A1 and SOX9 expression. Bar graphs present relative mRNA expression levels with statistical significance indicated. The cartilage-differentiation effect of seven drugs selected from the first screening was re-evaluated by measuring changes in mRNA levels of COL2A1, SOX9, and aggrecan (ACAN).: * P < 0.05, ** P < 0.01, *** P < 0.001 ( n = 3). ( C ) ADMSCs were cultured in pellet form and treated with DMSO, aripiprazole, or irinotecan for three weeks. Aripiprazole treatment showed increased cartilage differentiation compared to DMSO and irinotecan. ( D ) Hematoxylin and Eosin (H&E) staining and Alcian blue staining of pellet cultures. Aripiprazole treatment resulted in larger pellet size and more intense staining, indicating enhanced cartilage matrix production. Scale bars represent 100 μm.

    Journal: Scientific Reports

    Article Title: Therapeutic role of aripiprazole in cartilage defects explored through a drug repurposing approach

    doi: 10.1038/s41598-024-82177-1

    Figure Lengend Snippet: Functional screening of drugs promoting chondrogenic differentiation ( A ) First Screening of Cartilage Differentiation-Promoting Drugs: Adipose-derived mesenchymal stem cells (ADMSCs) were treated with 10 µM of each drug for 72 h. The expression levels of COL2A1 (top) and SOX9 (bottom) mRNA were assessed using qRT-PCR. Dotted lines indicate drugs that significantly increased SOX9 and COL2A1 expression compared to DMSO control. ( B ) Second Screening: The Venn diagram shows the overlap of drugs that increased COL2A1 and SOX9 expression. Bar graphs present relative mRNA expression levels with statistical significance indicated. The cartilage-differentiation effect of seven drugs selected from the first screening was re-evaluated by measuring changes in mRNA levels of COL2A1, SOX9, and aggrecan (ACAN).: * P < 0.05, ** P < 0.01, *** P < 0.001 ( n = 3). ( C ) ADMSCs were cultured in pellet form and treated with DMSO, aripiprazole, or irinotecan for three weeks. Aripiprazole treatment showed increased cartilage differentiation compared to DMSO and irinotecan. ( D ) Hematoxylin and Eosin (H&E) staining and Alcian blue staining of pellet cultures. Aripiprazole treatment resulted in larger pellet size and more intense staining, indicating enhanced cartilage matrix production. Scale bars represent 100 μm.

    Article Snippet: Human adipose tissue-derived mesenchymal stem cells (ADMSCs) were obtained from PromoCell GmbH (Heidelberg, Germany), ADMSCs were cultured in standard culture and chondrogenic differentiation media (Gibco).

    Techniques: Functional Assay, Derivative Assay, Expressing, Quantitative RT-PCR, Control, Cell Culture, Staining